| Date | 1882 |
| Author | Koch |
| Contribution | c. Used heat to get methylene blue into waxy envelope of tubercle bacilli so as to stain them. |
| Reference | Anon. |
| Notes | See Weigert; see Erhlich. Brock (1999) gives information on Koch's use of methylene blue but does not seem to mention heating. |
| Category | Miscellany |
| Pathogen Class | Bacteria |
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