| Date | 1875 |
| Author | Anon. |
| Contribution | Introduced "differentiation" to the staining of tissues. |
| Reference | Anon. |
| Notes | The date is an approximation, and no individual is known to have been solely responsible, but at about this time, "differentiation" was introduced to microscopical stain technology. It refers to the removal of excess stain by immersion of stained tissue in ethanol. |
| Category | Microscopy |
| Pathogen Class | General |
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