| Date | 1710 |
| Author | Joblot |
| Contribution | Boiled infusions remain free of microorganisms. |
| Reference | Lechevalier |
| Notes | In a controlled experiment, Joblot showed that animalcules did not appear in hay infusions that had been boiled and kept in a sterile closed container. This was a contribution to the long Spontaneous Generation controversy that was separate from, but relevant to, the Germ Theory. |
| Category | Miscellany |
| Pathogen Class | General |
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