| Date | 1882 |
| Author | Ziehl |
| Contribution | Used phenol (instead of Ehrlich's aniline water) with methyl violet for staining tubercle bacilli. |
| Reference | Brock |
| Notes | See 1883. This was the first component of the classic Ziehl-Neelsen stain. Ehrlich's aniline had previously replaced Koch's ammonia as an alkali (alkalinity being necessary for the successful staining of bacteria with methylene blue). |
| Category | Miscellany |
| Pathogen Class | Bacteria |
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