| Date | 1873 |
| Author | Tolles |
| Contribution | Designed and made a homogeneous immersion system. |
| Reference | Bradbury |
| Notes | He used thinned (soft) Canada balsam as the immersion fluid (the refractive index of the balsam was approximately the same as that of the cover glass). This was apparently the first practical homogeneous immersion system, but balsam was not as suitable as the oil introduced by Abbe; see 1879. |
| Category | Microscopy |
| Pathogen Class | General |
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