| Date | 1875 |
| Author | Koch |
| Contribution | Developed method for culture of anthrax bacilli, and observed complete spore formation. |
| Reference | Brock |
| Notes | In the process of inoculating a rabbit intraocularly with tissue from an anthrax-infected rabbit, Koch observed the suitability of aqueous humor for culture of anthrax bacilli. He also found that cultures on slides did especially well if maintained at 30-35 C, and if kept from drying yet not deprived of air. |
| Category | Causation |
| Pathogen Class | Bacteria |
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